abstract：:Based on the synchronization between a periodic vibration and flashing light, we designed a device to determine the flagellar beating frequency of human sperm. The head of a spermatozoon was either held by the tip of a micropipette operated with a micromanipulator or adhered by itself on the surface of a glass slide w...

journal_title：Andrologia

authors： Chen CS,Chao HT,Leng CH,Pan RL,Wei YH

更新日期：1998-02-01 00:00:00

abstract：:The presence of 2 million or more peroxidase-positive white blood cells per ml of semen, or the diagnosis of male accessory gland infection, is associated with important biochemical and biological changes in semen plasma and in the spermatozoa, reducing their fertilizing potential in vitro and in vivo (e.g., during in...

journal_title：Andrologia

authors： Depuydt C,Zalata A,Christophe A,Mahmoud A,Comhaire F

更新日期：1998-01-01 00:00:00

abstract：:The influence of bacteria and/or leukocytes on the outcome of IVF or ICSI is influenced by three factors which have little in common with in vivo conditions: 1) The process of ejaculate preparation (swim-up, Percoll) with antibiotic buffered media; 2) The small amount of inseminated spermatozoa (100,000 per culture); ...

journal_title：Andrologia

authors： Michelmann HW

更新日期：1998-01-01 00:00:00

abstract：:In order to investigate the role of testosterone propionate (TP) on the antioxidant system of the rat testis, lipid peroxidation (LPX) and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of the testis of testosterone-treated and control rats were compared. The results indicate...

journal_title：Andrologia

authors： Chainy GB,Samantaray S,Samanta L

更新日期：1997-11-01 00:00:00

abstract：:Parameters were obtained from the reproductive organs of ethanol-fed, gossypol-treated Sprague Dawley rats. The experimental animals were fed either on a normal (15.17%) or low protein (8.00%) diet. Measurements included reproductive organ weights, seminal characteristics, serum concentration of testosterone and histo...

journal_title：Andrologia

authors： Akingbemi BT,Rao PV,Aire TA

更新日期：1997-07-01 00:00:00

abstract：:The testis of the donkey was used as a model to study age-related changes in the period between puberty and senium. From the age of 1.5 years to the middle of sexual maturity (5 to 6 years) a number of histophysiological features, all indicative of the spermatogenetic efficiency, increase continuously. Then without a ...

journal_title：Andrologia

authors： Nipken C,Wrobel KH

更新日期：1997-05-01 00:00:00

abstract：:We have studied the response of atrophic Leydig cells to gonadotrophin replacement in young hypophysectomized (HX) and GnRH antagonist (GnRH-ANT)-treated rats. Hypophysectomy was performed at 28 days of age. Age-matched rats were treated with GnRH-ANT from 28 to 51 days of age. From 45 to 51 days of age, animals were ...

journal_title：Andrologia

authors： Gaytan F,Bellido C,Morales C,Aguilar E

更新日期：1997-03-01 00:00:00

abstract：:In order to correlate functional and biochemical parameters, frozen bovine sperm samples presenting diverse motility were studied. Specimens collected from four bulls (A, B, C and D) were graded according to motile spermatozoa percentage and vigour, regarding the sample from bull A as highest motility, those from bull...

journal_title：Andrologia

authors： Beorlegui N,Cetica P,Trinchero G,Córdoba M,Beconi M

更新日期：1997-01-01 00:00:00

abstract：:The role of N-methyl-D-aspartate (NMDA) and non-NMDA receptors in the control of FSH and LH secretion was analysed in prepubertal male rats. In the first experiment 4, 8, 12, 16, 20 and 30-day-old males were decapitated 15 min after vehicle, NMDA or kainic acid (KA) administration. In the second experiment, 23-day-old...

journal_title：Andrologia

authors： Aguilar E,Tena-Sempere M,Gonzalez D,Pinilla L

更新日期：1996-05-01 00:00:00

abstract：:The adult human Sertoli cells produced lactate, estradiol-17beta, transferrin and inhibin; germ cells modulate synthesis of these compounds. In order to study the functional features of human Sertoli cells in vitro, the aim of this study was to measure the lactic dehydrogenase (LDH), gamma-glutamyl transpeptidase (GGT...

journal_title：Andrologia

authors： Carreau S,Drosdowsky MA,Foucault P

更新日期：1996-03-01 00:00:00

abstract：:The ZP3 protein is a zona pellucida glycoprotein which plays a major role in sperm binding and induction of the acrosome reaction. ZP3 proteins occur in various mammalian zonae pellucidae; their primary structures are highly conserved as revealed by cDNA cloning. We generated antisera against synthetic peptides that a...

journal_title：Andrologia

authors： Hinsch E,Hinsch KD

更新日期：1996-01-01 00:00:00

abstract：:Microsurgical epididymal sperm aspiration (MESA) and testicular sperm extraction (TESE) require close cooperation between andrologists, urologists and gynaecologists. Intracytoplasmic sperm injections were established in Giessen in March 1994 and embryo transfer (ET) was performed in 342 of 375 patients (91.2%). The p...

journal_title：Andrologia

authors： Köhn FM,Schroeder-Printzen I,el Mulla KF,Jung A,Stalf T,Weidner W,Gips H,Künzel W,Schill WB

更新日期：1996-01-01 00:00:00

abstract：:Sperm cell plasma membrane and the outer acrosomal membrane fuse profusely during the acrosome reaction. The process is triggered by extracellular signals that elicit several intracellular events leading ultimately to membrane fusion. We have developed a streptolysin O permeabilizing protocol that selectively affects ...

journal_title：Andrologia

authors： Díaz A,Domínguez L,Fornés MW,Burgos MH,Mayorga LS

更新日期：1996-01-01 00:00:00

abstract：:Little is known about the biological and clinical significance of bilaterally enlarged testes (bilateral megalotestes or macroorchidism). We have investigated clinical, endocrine and spermatological characteristics of 135 andrological patients with a testicular volume of more than 25 ml bilaterally. 135 patients with ...

journal_title：Andrologia

authors： Meschede D,Behre HM,Nieschlag E

更新日期：1995-07-01 00:00:00

abstract：:EPON labelled with bromide was used to embed ejaculated rabbit spermatozoa, with the hypothesis that it replaces cell water. X-Ray spectrophotometric microanalysis of sperm nuclei, of egg yolk (an internal standard containing roughly 50% water) and of surrounding embedding resin, revealed that a part of the bromide wa...

journal_title：Andrologia

authors： Ekwall H,Plöen L,Courtens JL

更新日期：1995-05-01 00:00:00

abstract：:E prostaglandins are formed in seminal vesicles and can be oxygenated by (omega-1)-hydroxylation catalysed by cytochrome P450 to 19(R)-hydroxy metabolites. The latter are not further metabolized. Prostaglandin E1 (PGE1), PGE2, 19-hydroxy-PGE1 and 19-hydroxy-PGE2 were measured in seminal fluid of 95 men, who attended t...

journal_title：Andrologia

authors： Pourian MR,Kvist U,Björndahl L,Oliw EH

更新日期：1995-03-01 00:00:00

abstract：:The scope of this study was to evaluate the accuracy, precision and specificity of the sperm concentration measurements by the Strömberg-Mika Cell Motion Analyser (SM-CMA). Our data show that the instrument generally underscores the sperm concentration and therefore the uncorrected measurements must be corrected by th...

journal_title：Andrologia

authors： Togni G,Gallicciotti G,Coccia P,Piffaretti-Yanez A,Stamm J,Balerna M

更新日期：1995-01-01 00:00:00

abstract：:D-chloramphenicol, at concentrations of 20 and 40 micrograms/ml, inhibited over 80% of the newly synthesized mitochondrial proteins expressed by incorporation of 3H-amino acid mixture. At concentrations of 20 and 40 micrograms ml-1, D-chloramphenicol enhanced the collective motility of washed ram spermatozoa. The coll...

journal_title：Andrologia

authors： Twaina-Bechor E,Bartoov B

更新日期：1994-11-01 00:00:00

abstract：:The microvasculature of the human testis is closely related to the Leydig cells and the seminiferous tubules. Semi-thin sections of testicular tissue serve as a basis for the computer-aided 3-D reconstruction of the microvasculature, the seminiferous tubules and the Leydig cells. After vascular perfusion with glutaral...

journal_title：Andrologia

authors： Ergün S,Stingl J,Holstein AF

更新日期：1994-09-01 00:00:00

abstract：:Recent studies have revealed a high prevalence of leukocytospermia (> 1 x 10(6) white blood cells ml-1 semen) in male infertility patients in the USA and certain European countries, and have implicated white blood cells as a cause of infertility. Since leukocytospermia may often be attributed to male genital-tract inf...

journal_title：Andrologia

authors： Wang AW,Politch J,Anderson D

更新日期：1994-05-01 00:00:00

abstract：:The study described here was carried out to establish whether or not chloroquine was excreted in semen. Chloroquine was assayed in the semen of eight male volunteers (aged between 19 and 45 years) by a fluorimetric method before and after taking a total dose of 1.500 mg chloroquine base corresponding to the accepted d...

journal_title：Andrologia

authors： Adeeko AO,Dada OA

更新日期：1994-05-01 00:00:00

abstract：:The sperm function of fertile men (control), infertility patients (experimental), and men with varicocele were compared. The bioassays used were the follicular fluid-induced acrosome reaction, the binding to the zona pellucida, and the penetration of zona-free hamster oocytes. The percentage (mean +/- SEM) of reacted ...

journal_title：Andrologia

authors： Vigil P,Wöhler C,Bustos-Obregón E,Comhaire F,Morales P

更新日期：1994-03-01 00:00:00

abstract：:The role of opioidergic system in controlling gonadotropin secretion in elderly men still remains uncertain. In the present study, we attempted to examine the opioidergic inhibitory tone imposed on GnRH release mechanism in elderly men by monitoring the plasma gonadotropin response to the opiate receptor antagonist na...

journal_title：Andrologia

authors： Mikuma N,Kumamoto Y,Maruta H,Nitta T

更新日期：1994-01-01 00:00:00

abstract：:Several drugs have been used to stimulate human sperm motility, including 3-deoxy-adenosine, caffeine, and pentoxifylline. Pentoxifylline is an inhibitor of the phosphodiesterase and may stimulate sperm motility by increasing the intracellular levels of cAMP. In this study we have evaluated the effect of pentoxifyllin...

journal_title：Andrologia

authors： Morales P,Llanos M,Yovich JL,Cummins JM,Vigil P

更新日期：1993-11-01 00:00:00

abstract：:A procedure was designed for purification of a human seminal plasma-specific antigen (HSP-antigen) identified by a monoclonal antibody produced in this laboratory (mAb 4E6). Pooled human seminal plasma was fractionated by consecutively applied methods: affinity chromatography on Lentil lectin sepharose, gel chromatogr...

journal_title：Andrologia

authors： Dimitrova D,Kehayov I,Kyurkchiev S

更新日期：1993-09-01 00:00:00

abstract：:The possible physiological role of testicular corticotropin-releasing factor (CRF) in the regulation of testicular functions was studied in neonatal rats. Two microlitres of anti-CRF-antiserum (dilution: 1:10 or 1:100) was injected intratesticularly to 5 d-old rats with two testes and to hemicastrates. Five days after...

journal_title：Andrologia

authors： Gerendai I,Nemeskéri A,Csernus V

更新日期：1993-07-01 00:00:00

abstract：:Two monoclonal antibodies (MAbs) directed against human protamine P1 were realized. Anti-P1 specificity was assessed by western-blot and confirmed by ELISA. Monoclonal antibody 97-3 was selected. Protamine P1 was specifically demonstrated in human testis by immunoelectron microscopy, using 97-3 MAb and an indirect pos...

journal_title：Andrologia

authors： Lescoat D,Blanchard Y,Lavault MT,Quernée D,Le Lannou D

更新日期：1993-03-01 00:00:00

abstract：:Twenty infertile patients with normal tubal patency were inseminated intraperitoneally (11 once, seven twice, and two three times) with spermatozoa (mean 14 x 10(6), range 0.6-48 x 10(6)) prepared by the standard swim-up technique. The occurrence of immunization to spermatozoa was looked for by the Gelatin Agglutinati...

journal_title：Andrologia

authors： Ragni G,Lenzi A,Gandini L,Cristiani C,Lombroso GC,Olivares MD,Wyssling H,Dondero F,Crosignani PG

更新日期：1993-01-01 00:00:00

abstract：:Primordial germ cells in the mouse are known to be derived from the epiblast. They can be identified histochemically, by their high alkaline phosphatase activity. At 8 d post coitum they have been observed within the embryonic part of the egg cylinder, at the posterior end of the primitive streak. Earlier, at 7.25 day...

journal_title：Andrologia

authors： McLaren A

更新日期：1992-09-01 00:00:00

abstract：:DNA specific fluorochrome (Hoechst 33342 and 33258) as non-toxic stains, have been widely used to measure cell density and proliferation, detect sperm-egg fusion, and observe the development of pre-implantation embryos. It has been reported that Hoechst 33342 at a concentration of 10 micrograms ml-1 had significant in...

journal_title：Andrologia

authors： Yelian FD,Dukelow WR

更新日期：1992-05-01 00:00:00

abstract：:In this randomized prospective study, we determined the conception rate following intra-uterine insemination with washed and prepared sperm, or with the first portion of a split ejaculate, in couples with longstanding male (n = 27, 70 treatment cycles) or cervical infertility (n = 14, 29 treatment cycles). Folliculoge...

journal_title：Andrologia

authors： Goldenberg M,Rabinovici J,Bider D,Lunenfeld B,Blankstein J,Weissenberg R

更新日期：1992-05-01 00:00:00

abstract：:Flutamide, a pure antiandrogen, increases the levels of plasma luteinizing hormone but antagonizes the biological expression of androgen on target organs. Flutamide was administered to rats to study the effect of altered availability of hormones on the functional status of epididymis. The weights of ventral prostate, ...

journal_title：Andrologia

authors： Mukherjee M,Chattopadhyay S,Mathur PP

更新日期：1992-03-01 00:00:00

abstract：:We fractionated the whole human seminal plasma on DEAE Sephadex A-50 ion exchange columns. Complete separation was achieved in seven peaks using different salt concentrations in phosphate buffer pH 6. The seminal plasma proteins were separated by sodium dodecyl-sulphate polyacrylamide gel electrophoresis. Human semina...

journal_title：Andrologia

authors： Haq A,al-Tufail M,Sheth K,Abdullatif M,Hamilton C,al-Abdul Jabbar F,al-Sedairy S

更新日期：1992-03-01 00:00:00

abstract：:Structural heterozygosities of the karyotype have detrimental effects on the meiotic process, resulting very often in impairment of fertility in the carriers. Both male and female germ cell development are affected by chromosomal variability although spermatogenesis seems particularly prone to be affected, probably be...

journal_title：Andrologia

authors： Redi CA,Garagna S

更新日期：1992-01-01 00:00:00

abstract：:The spontaneous acrosomal reaction of mouse spermatozoa during their passage through the male and female genital tract was studied by indirect immunofluorescence staining techniques using antibodies against the outer acrosomal membrane (OAM). Low rates of spontaneously acrosome-reacted spermatozoa could be observed du...

journal_title：Andrologia

authors： Klemm M,Engel W

更新日期：1991-11-01 00:00:00

abstract：:This study was set to determine if there is a correlation between the general toxic effect and the gonadotoxic effect of DBCP on male rats. Groups of male rats were injected with a single dose of DBCP (50 mg kg-1) dissolved in dimethylsulfoxide (DMSO). Twenty four hours, one and four weeks post injection animals were ...

journal_title：Andrologia

authors： Kaplanski J,Shemi D,Waksman J,Potashnik G,Sod-Moriah UA

更新日期：1991-09-01 00:00:00

abstract：:After a single injection of serum gonadotrophin (PMSG) at the dose of 15 IU/kg, i.m. into rams testosterone in the plasma of blood showed a significant rise between 4th and 7th day post-injection. At the same time (4th-7th day) the plasminogen activator activity (PAA) in seminal plasma was found to be increased, but t...

journal_title：Andrologia

authors： Rekkas C,Belibasaki S,Taitzoglou I,Kokolis N,Smokovitis A

更新日期：1991-07-01 00:00:00

abstract：:Angiotensin converting enzyme (EC 3.4.15.1) from bovine seminal plasma was shown to exist in two distinct forms. A lower molecular weight form of the enzyme having higher activity was purified to homogeneity. Final recovery of the enzyme was 18.37%. The apparent molecular weight of the enzyme was estimated to be 1.99 ...

journal_title：Andrologia

authors： Sharma M,Singh US

更新日期：1991-07-01 00:00:00

abstract：:Epidermal growth factor concentrations and amounts per ejaculate were determined in 162 infertile men and in a control group of 50 pregnancy-proven fertile men. No differences were found between those groups. The group of infertile patients was divided into sub-groups according to their sperm density. EGF values in th...

journal_title：Andrologia

authors： Delgado SR,Ramirez K,Mallea E

更新日期：1991-05-01 00:00:00

abstract：:The acrosome reaction of human spermatozoa was induced by changes of temperature. Spermatozoa were collected from fertile donors and a patient group, and selected by the "swim-up" method. The spermatozoa were treated in two different ways: Protocol I: 24 hours at room temperature followed by additional incubation at 3...

journal_title：Andrologia

authors： Sanchez R,Toepfer-Petersen E,Aitken RJ,Schill WB

更新日期：1991-05-01 00:00:00